Resveratrol enhances apoptosis induced by the heterocyclic aromatic amines in p53-wt LoVo cells, but not in p53-deficient HaCaT cells
Katarzyna Kuryłowicz
Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
https://orcid.org/0000-0003-1456-8097
Agnieszka Cierniak
Department of Medicine and Health Sciences, Andrzej Frycz Modrzewski Krakow, University, Kraków, Poland
https://orcid.org/0000-0001-6537-2954
Ewelina Madej
Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
https://orcid.org/0000-0002-0729-0245
Agnieszka Wolnicka-Głubisz
Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
https://orcid.org/0000-0003-3070-6349
In the present study, we investigated the influence of resveratrol on PhIP treated human colon cancer cells and compared the effect to HaCaT cells considered as normal, human keratinocytes. Our results show that resveratrol decreases DNA damage in both cell types, it increases the sensitivity of LoVo cells to apoptosis and has no effect on PhIP-treated HaCaT cells. We confirm that PhIP-induced apoptosis is p53 and caspase 3/7 dependent. Interestingly, normal cells such as HaCaT, which lack functional p53 are more resistant to PhIP treatment.
Author Biographies
Agnieszka Cierniak, Department of Medicine and Health Sciences, Andrzej Frycz Modrzewski Krakow, University, Kraków, Poland
Department of Medicine and Health Sciences, Andrzej Frycz Modrzewski Krakow University, Herlinga-Grudzińskiego 1, 30-705 Krakow, Poland
Ewelina Madej, Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland
Łukasz Skalniak, Faculty of Chemistry, Jagiellonian University, Kraków, Poland
Faculty of Chemistry, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland
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