The expression levels of NF-κB and IKKβ in epithelial ovarian cancer and their correlation with drug resistance-related genes MDR1, TOPOII, and ERCC1

  • Zhexia Hu Department of Gynaecology and Obstetrics, The Fourth Hospital of Shijiazhuang, Shijiazhuang, Hebei, China
  • Rong Cang Department of Gynecological Endocrinology, The Fourth Hospital of Shijiazhuang, Shijiazhuang, Hebei, China
  • Hui Zhang Department of Gynecology and Obstetrics, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China
  • Yu Gao Department of TCM, The Fourth Hospital of Shijiazhuang, Shijiazhuang, Hebei, China
  • Xueling Zhang Department of Gynaecology and Obstetrics, The Fourth Hospital of Shijiazhuang, Shijiazhuang, Hebei, China
  • Xiao Chen Department of Gynaecology and Obstetrics, The Fourth Hospital of Shijiazhuang, Shijiazhuang, Hebei, China
  • Yun Rong Department of Gynaecology and Obstetrics, The Fourth Hospital of Shijiazhuang, Shijiazhuang, Hebei, China
  • Biyun Luo Department of Gynaecology and Obstetrics, The Fourth Hospital of Shijiazhuang, Shijiazhuang, Hebei, China
  • Yingfang Gao Department of Gynaecology and Obstetrics, The Fourth Hospital of Shijiazhuang, Shijiazhuang, Hebei, China https://orcid.org/0000-0002-7516-6713
DOI: https://doi.org/10.18388/abp.2020_5727

Abstract

Objectives: To explore the expression levels of nuclear factor kappa B (NF-κB) and inhibitor of nuclear factor kappa B kinase (IKKβ) in epithelial ovarian cancer and the correlation analysis with multi-drug resistance-related genes 1 (MDR1), topoisomerase II (TOPOII) and nucleotide excision repair cross complementary group 1 (ERCC1). Methods: Immunohistochemical methods were used to detect the expression levels of NF-κB and IKKβ in epithelial ovarian cancer group (50 cases), ovarian benign tumor group (30 cases), and normal ovary group (10 cases). The expression levels of NF-κB, IKKβ, MDR1, TOPOII and ERCC1 messenger ribonucleic acid (mRNA) and protein were analyzed using real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. Student’s t-test and one-way ANOVA were used for comparison of numerical data. Pearson’s chi-squared and Fisher’s exact tests were carried out for analysis of non-numerical data. Results: The levels of NF-κB, IKKβ, MDR1 and ERCC1 mRNA and protein were increased (P<0.05), and the expression levels of TOPOII were decreased (P<0.05) in the epithelial ovarian cancer group compared to the normal ovary and benign ovarian tumor groups. The expression of NF-κB and IKKβ in epithelial ovarian cancer was significantly increased in patients with higher tumor stage, lower differentiation and presence of lymph node metastasis and positively correlated with MDR1 expression. NF-κB and IKKβ were negatively correlated with the expression of TOPOII and antagonized each other with TOPOII. Conclusions: The expression of NF-κB and IKKβ was positively correlated with the expression of MDR1, and negatively correlated with the expression of TOPOII. The correlation of NF-κB, IKKβ and resistance related genes, including MDR1, TOPOII, ERCC1, can predict the resistance of chemotherapy individuals to chemotherapy.

Published
2023-02-17
Issue
Vol. 70 No. 1 (2023)
Section
Articles

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