Evaluating the antibacterial activity of muramyl dipeptide derivatives, retro-tuftsin derivatives, and anthraquinone oligopeptides against a range of pathogenic bacteria

Magdalena Wysocka; Krystyna Dzierzbicka; Beata Krawczyk

doi:10.18388/abp.2020_5757

Magdalena Wysocka Gdansk University of Technology, Faculty of Chemistry, Department of Molecular Biotechnology and Microbiology, Gdańsk, Poland https://orcid.org/0000-0001-9859-7068
Krystyna Dzierzbicka Gdansk University of Technology, Faculty of Chemistry, Department of Organic Chemistry, Gdańsk, Poland
Beata Krawczyk Gdansk University of Technology, Faculty of Chemistry, Department of Molecular Biotechnology and Microbiology, Gdańsk, Poland https://orcid.org/0000-0001-5528-8898

DOI: https://doi.org/10.18388/abp.2020_5757

Abstract

Search for new and efficient antibiotic is crucial because of microbial drug resistance and problems with side effects of the administered medication. In this study, we evaluate the in vitro microbiological activity of muramyl dipeptide derivatives, retro-tuftsin derivatives (i.e., tuftsin with reversed amino acid sequences), and combinations of retro-tuftsin derivatives with substituted anthraquinones. The potency of the investigated derivatives towards methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae ESBL (extended-spectrum β-lactamases) was compared based on the spectroscopically-measured minimal inhibitory concentrations (MIC values). The bacterial growth have also been studied with different concentrations of compounds. Statistical analysis of the results revealed that certain modifications lead to promising activity against S. aureus (anthraquinone analogue – 3c and retro-tuftsin derivative – 2b), while other derivatives exhibit activity against P. aeruginosa (muramyl dipeptide derivative – 1d and retro-tuftsin derivative – 2b). The obtained results of microbiological activity indicate that the structure of the tested compounds may be the basis for further modifications.