LINC00313/miR-4429 axis provides novel biomarkers for the diagnosis and prognosis of non-small cell lung cancer

Jinxiang Wang; Lihua Zhang; Caihong Wang; Yanfang Chen; Xiaomei Sui

doi:10.18388/abp.2020_5794

Jinxiang Wang Department of Radiotherapy, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong, China
Lihua Zhang Department of Radiotherapy, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong, China
Caihong Wang Department of Radiotherapy, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong, China
Yanfang Chen Department of Radiotherapy, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong, China
Xiaomei Sui Department of Radiotherapy, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong, China

DOI: https://doi.org/10.18388/abp.2020_5794

Abstract

Background: Increasing evidence suggests that the LncRNA/miRNA axis plays a key role in many types of tumorigenesis. However, the potential role and clinical value of LINC00313/miR-4429 in non-small cell lung cancer (NSCLC) remain elusive and need further study. The aim of this study was to investigate the role of LINC00313/miR-4429 axis in NSCLC. Methods: The expression of LINC00313 and miR-4429 in serum, tissues and cell lines of NSCLC patients were detected by reverse transcription-quantitative PCR. The diagnostic value was evaluated by receiver operating characteristic (ROC) curve analysis and the prognostic value was analyzed by Kaplan-Meier survival analysis and Cox regression. Result: The expression of LINC00313 was significantly up-regulated while the expression of miR-4429 was down-regulated in NSCLC serum, tissue and cell lines. A negative correlation was found between LINC00313 and miR-4429 in patients with NSCLC. ROC curves showed that LINC00313 and miR-4429 had high diagnostic value and Kaplan-Meier curve results showed that high expression of LINC00313 and low expression of miR-4429 predicted poor prognosis. Conclusion: In summary, our data show that the ectopic expression of LINC00313 and miR-4429 has promising clinical significance in the diagnosis and prognosis of NSCLC. The LINC00313/miR-4429 axis may provide novel biomarker for NSCLC treatment.