Long non-coding RNA MINCR regulates the growth and metastasis of human osteosarcoma cells via Wnt/β-catenin signaling pathway

  • Sunpeng Bai Department of Orthopedics, The First People's Hospital of Tianmen In Hubei Province, Tianmen, Hubei, China, 431700Department of Orthopedics, The First People’s Hospital of Tianmen In Hubei Province, Tianmen, Hubei, 431700, China
  • Yongjiang Li Department of Orthopedics, The First People’s Hospital of Tianmen In Hubei Province, Tianmen, Hubei, 431700, China
  • Yuancheng Wang Department of Orthopedics, The First People’s Hospital of Tianmen In Hubei Province, Tianmen, Hubei, 431700, China
  • Gaoli Zhou Department of Orthopedics, The First People’s Hospital of Tianmen In Hubei Province, Tianmen, Hubei, 431700, China
  • Cheng Liu Department of Orthopedics, The First People’s Hospital of Tianmen In Hubei Province, Tianmen, Hubei, 431700, China
  • Wen Xiong Department of Orthopedics, The First People’s Hospital of Tianmen In Hubei Province, Tianmen, Hubei, 431700, China
  • Junfeng Chen Department of Orthopedics, The First People’s Hospital of Tianmen In Hubei Province, Tianmen, Hubei, 431700, China
DOI: https://doi.org/10.18388/abp.2020_5804

Abstract

There is concrete evidence that lncRNA-MINCR is involved in tumorigenesis of a number of human cancers through modulation of Wnt/β-catenin signaling pathway. However, the characterization of regulatory role of lncRNA-MINCR has not been worked out in osteosarcoma yet. The present study was undertaken to explore the role of lncRNA-MINCR in human osteosarcoma. The osteosarcoma tissues and cell lines were found to exhibit significant (P<0.05) over-expression of lncRNA-MINCR. Silencing of lncRNA-MINCR in osteosarcoma cells suppressed their cell viability through the induction of apoptosis. The Saos-2 osteosarcoma cells exhibited significant (P<0.05) decline in migration and invasion rate together with inhibition of EMT under transcriptional knockdown of lncRNA-MINCR. Western blot analysis revealed that lncRNA-MINCR operated through Wnt/β-catenin signaling pathway to control the growth and metastasis of osteosarcoma cells. In vivo mice tumorigenesis was significantly (P<0.05) restricted under lncRNA-MINCR repression. The study clearly indicated that lncRNA-MINCR exhibits crucial growth regulatory role in osteosarcoma together with its ability to control the metastasis of cancer cells through Wnt/β-catenin signal.

Published
2022-06-29
Issue
Vol. 69 No. 3 (2022)
Section
Articles

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