Effect of miR-129-3p on autophagy of interstitial cells of Cajal in slow transit constipation through SCF C-kit signaling pathway

  • Heng Wang 1Department of Pediatric Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China; 2Department of Gastrointestinal Surgery/ Pediatric Surgery, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China
  • Bingbing Ren Department of Pediatric Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China
  • Jun Pan Department of Traditional Chinese Medicine, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China
  • Siqi Fu Department of Pediatric Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China
  • Chunxiang Liu Department of Pediatric Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China
  • Daqing Sun Department of Pediatric Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China
DOI: https://doi.org/10.18388/abp.2020_5877

Abstract

Objective: To explore the mechanism by which miR-129-3p affected the autophagy of interstitial cells of Cajal (ICCs) in slow transit constipation tissues through the SCF C-kit signaling pathway. Methods: Colon samples from 20 Slow transit constipation (STC) patients who underwent total colectomy plus ileorectal anastomosis or subtotal colon resection plus anti-peristaltic rectal anastomosis were collected in our hospital. The colon of 20 non-STC patients was used as control. The control of this study was 20 patients undergoing radical surgery for colon cancer (left colon cancer) in our hospital. Fifty healthy SPF Kunming mice were purchased from Liaoning Changsheng Biotechnology Co., Ltd. Results: The mRNA expression of miR-129-3p in the STC group was lower than that in the control group (CTLR) group (P<0.05). The mRNA expression of miR-129-3p in STC group was lower than that in the NC group (P<0.05), and mRNA expression in STC+miR-129-3p group was higher than that in STC+miR-NC group (P<0.05). In the first week, the weight of dry and wet feces of the STC group was lower than that of the NC mice (P<0.05), and the weight of dry feces and wet feces of the STC group was lower than that of the NC group at the 2, 3, and 4 weeks, STC+miR-129 -3p was higher than that in the STC group (P<0.05). Conclusion: The increased expression of C-kit and SCF regulated by miR-129-3p contributed to the protection of interstitial cells. Knockdown of miR-129-3p expression could inhibit the activation of AKT/mTOR signaling pathway, reduce cell proliferation activity.

Published
2022-09-04
Issue
Vol. 69 No. 3 (2022)
Section
Articles

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