MicroRNA-15 suppresses viability, migration and invasion of the human MG-63 osteosarcoma cells via inhibition of cyclin dependent kinase 6 (CDK6)

Zhao Shen; Chen Wang; Bin Liu; Jieli Du; Zhiqiang Li; Jitang Zhao

doi:10.18388/abp.2020_6001

Zhao Shen Department of Orthopedics, Cangzhou People’s Hospital, Cangzhou, Hebei, China, 061000
Chen Wang Department of Orthopedics, Cangzhou People’s Hospital, Cangzhou, Hebei, China, 061000
Bin Liu Department of Orthopedics, Cangzhou People’s Hospital, Cangzhou, Hebei, China, 061000
Jieli Du Department of Orthopedics, Cangzhou People’s Hospital, Cangzhou, Hebei, China, 061000
Zhiqiang Li Department of Orthopedics, Cangzhou People’s Hospital, Cangzhou, Hebei, China, 061000
Jitang Zhao Department of Orthopedics, Cangzhou People’s Hospital, Cangzhou, Hebei, China, 061000

DOI: https://doi.org/10.18388/abp.2020_6001

Abstract

MicroRNA-15a-3p (miR-15) acts as tumor-suppressor in different human cancers including osteosarcoma. Nonetheless, the molecular function of miR-15 in osteosarcoma via suppression of cyclin dependent kinase 6 (CDK6) is yet to be studied. The results showed significant downregulation of miR-15 in osteosarcoma tissues and cell lines. Overexpression of miR-15 inhibited the proliferation and colony formation of the MG-63 osteosarcoma cells via induction of apoptosis. Moreover, miR-15 inhibited the migration and invasion of MG-63 osteosarcoma cells. The tumor-suppressive functional role of miR-15 was shown to be exerted via suppression of CDK6. The expression of CDK6 was upregulated in osteosarcoma and its silencing could exert growth inhibitory effects on human osteosarcoma cells. However, overexpression of CDK6 could nullify the tumor-suppressive effects of miR-15 on the MG-63 osteosarcoma cells. Taken together, miR-15 negatively regulates growth, migration and invasion of osteosarcoma cells by targeting CDK6 at post-transcriptional level. These findings suggest the therapeutic potential of miR-15/CDK6 in human osteosarcoma.