Anticancer effects of zapotin flavone in human gastric carcinoma cells are mediated via targeting m-TOR/PI3K/AKT signalling pathway

  • Yunlan Chen Department of Cadre Medical, the Third Affiliated Hospital of Kunming Medical University and Yunnan Cancer Hospital, Kunming, Yunnan, China
  • Jiangqiong Han Department of Integrated Chinese and Western Medicine, the Third Affiliated Hospital of Kunming Medical University and Yunnan Cancer Hospital, Kunming, Yunnan, China
  • Guoqin Wang Biological Immunotherapy Center, the Third Affiliated Hospital of Kunming Medical University and Yunnan Cancer Hospital, Kunming, Yunnan, China
  • Bin Qian Department of Pharmacy, the First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
  • Hua Zhao Department of Pharmacy, Shaanxi Provincial Cancer Hospital, Xi’an, Shaanxi, China https://orcid.org/0000-0002-3780-7583
DOI: https://doi.org/10.18388/abp.2020_6076

Abstract

The current study aims to investigate the anticancer effects of zapotin flavone in human gastric carcinoma cells. MTT assay was performed to determine the cytotoxicity effects of zapotin against the gastric cancer cells (SNU-1) and normal gastric cells (GES-1). SNU-1 cell morphology was analyzed through phase-contrast microscopy. Apoptosis was identified through DAPI staining assay and quantified through annexin V/propidium iodide (PI) staining. The effects on cell migration and invasion were carried out through transwell assay. Apoptosis and m-TOR/PI3K/AKT signalling pathway related proteins were analysed through western blotting. Proliferation rate of gastric cancer SNU-1 cell line declined with enhanced zapotin concentrations in comparison to normal GES-1 cells. Substantial morphological changes after zapotin exposure, including nuclear condensation and membrane rupture was observed. Further, increasing number of apoptotic cells, suppression of both cell migration and invasion was observed with increased zapotin concentrations. Finally, western blotting indicated significant blocking of m-TOR/PI3K/AKT signalling pathway. We conclude that zapotin can act as a potential drug against the gastric cancer.

Published
2022-06-09
Issue
Vol. 69 No. 2 (2022)
Section
Articles

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