Circular RNA CSPP1 motivates renal cell carcinoma carcinogenesis and the Warburg effect by targeting RAC1 through microRNA-493-5p

  • Dong Zhang Department of Urology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an City, Shaanxi Province, 710004, China
  • XiaoJie Yang Department of Urology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an City, Shaanxi Province, 710004, China
  • QiDong Luo Department of Urology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an City, Shaanxi Province, 710004, China
  • DeLai Fu Department of Urology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an City, Shaanxi Province, 710004, China
  • HongLiang Li Department of Urology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an City, Shaanxi Province, 710004, China
  • Peng Zhang Department of Urology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an City, Shaanxi Province, 710004, China https://orcid.org/0000-0002-7222-085X
  • Chong Tie Department of Urology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an City, Shaanxi Province, 710004, China
DOI: https://doi.org/10.18388/abp.2020_6299

Abstract

Circular RNAs (circRNAs) take on regulatory roles in renal cell carcinoma (RCC). The research’s goal was to figure out circ-CSPP1’s role and molecular mechanism in RCC. The results clarified that circ-CSPP1 expression was enhanced in RCC. Down-regulating circ-CSPP1 refrained the proliferation, migration, invasion, and Warburg effect (aerobic glycolysis), but accelerated apoptosis of RCC cells. The luciferase activity assay exhibited that circ-CSPP1 could perform as an endogenous sponge for miR-493-5p. Elevating miR-493-5p repressed RCC progression. The bioinformatics website starBase confirmed that ras-related C3 botulinum toxin substrate 1 (RAC1) was a target gene of miR-493-5p. Circ-CSPP1 up-regulated RAC1 by sponging miR-493-5p, and elevating RAC1 could turn around the effect of down-regulating circ-CSPP1 on RCC cells. Taken together, circ-CSPP1 is identified as a novel RCC-promoting RNA that could serve as a latent therapeutic target for RCC therapy.

Published
2023-09-18
Issue
Vol. 70 No. 3 (2023)
Section
Articles

Copyright (c) 2023 Peng Zhang, Dong Zhang, XiaoJie Yang, QiDong Luo, DeLia Fu, HongLiang Li, Chong Tie

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