Simvastatin attenuates diabetes mellitus erectile dysfunction in rats by miR-9-5p-regulated PDCD4

  • YiMing Weng Department of Reproductive Center, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China
  • YuanShen Mao Department of Urology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 201999, China
  • YanQiu Wang Department of Reproductive Center, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China
  • YuFan Jiao Department of Reproductive Center, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China
  • Jun Xiang Department of Urology, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200092, China https://orcid.org/0000-0002-1747-9113
  • Wei Le Department of Urology, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200092, China
DOI: https://doi.org/10.18388/abp.2020_6315

Abstract

DMED is a common complication of diabetes, for which new treatment methods are urgently required. Focused on DMED, the pharmacological mechanism of simvastatin (Sim) was probed. A model of DMED was made in rats with streptozotocin and orally medicated with Sim. Lentiviral vectors that interfere with miR-9-5p or PDCD4 were injected, and the erectile function, histopathology of cavernous tissue, and α-SMA expression were evaluated. Cavernous smooth muscle cells (CMSCs) obtained from DMED rats were treated with Sim and transfected with the plasmid vector that interferes with miR-9-5p or PDCD4 to observe cell viability and apoptosis. The binding relationship between miR-9-5p and PDCD4 was checked. After 8-week treatment with Sim, erectile function was improved and the corpus cavernosum injury was alleviated. Upregulating miR-9-5p or downregulating PDCD4 further improved erectile function and cavernous injury in rats. miR-9-5p targeted regulation of PDCD4. In vitro cell experiment results showed that Sim induced proliferation and reduced apoptosis of CSMCs by enhancing miR-9-5p-targeted regulating PDCD4 in vitro. Sim attenuates DMED in rats via miR-9-5p/PDCD4.

Published
2023-11-06
Issue
Vol. 70 No. 4 (2023)
Section
Articles

Copyright (c) 2023 YiMing Weng, YuanShen Mao, YanQiu Wang, YuFan Jiao, Jun Xiang, Wei Le

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