Resveratrol inhibits multiple organ injury in preeclampsia rat model

Xiao  Lang; Wei Liu; Qinfang Chen; Xingyu Yang; Dan Chen; Weiwei Cheng

doi:10.18388/abp.2020_6331

Xiao Lang 1The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; 2Shanghai Municipal Key Clinical Specialty, Shanghai, China
Wei Liu 1The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; 2Shanghai Municipal Key Clinical Specialty, Shanghai, China
Qinfang Chen 1The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; 2Shanghai Municipal Key Clinical Specialty, Shanghai, China
Xingyu Yang 1The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; 2Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China
Dan Chen 1The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; 2Shanghai Municipal Key Clinical Specialty, Shanghai, China
Weiwei Cheng 1The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; 2Shanghai Municipal Key Clinical Specialty, Shanghai, China; 3Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China https://orcid.org/0000-0002-4478-5239

DOI: https://doi.org/10.18388/abp.2020_6331

Abstract

Objective: To investigate the effects of resveratrol on multiple organ injury and energy metabolism and its possible mechanism in preeclampsia model. Methods: A total of 45 clean-grade female adults Sprague Dawley (SD) rats (weight 270–320 g) were randomly divided into three groups: a control group, preeclampsia group (PE), and Preeclampsia with resveratrol treatment group (RE). Preeclampsia was induced in rats by administering 200 mg/kg/day L-NAME. Expression levels of TNF-α and IL-6 in the lungs and kidney tissues were analysed by ELISA, while the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) was determined by biochemical assays. The levels of lactic acid and pyruvate were detected using biochemical assays, while the epinephrine level in the kidney and heart tissues was determined by the ELISA method. Reverse transcription polymerase chain reaction and the western blotting were used to detect the expression of pyruvate dehydrogenase kinase 4 (PDK4) in the myocardial tissues. Results: We found that resveratrol treatment inhibited the levels of IL-6, TNF-α, and MDA in the lungs and kidney tissues, while the SOD activity was increased. Treatment with resveratrol reduced the levels of lactic acid, pyruvate, and epinephrine in the kidney and heart tissues. Furthermore, resveratrol treatment significantly increases the expression of PDK4 myocardial tissues in RE group compared to PE group. Conclusion: Resveratrol may inhibit the release of tissue inflammatory factors, regulate the body’s energy metabolism, and ultimately protect tissue damage caused during Preeclampsia.