Hepatoprotective effects of walnut oil and Caralluma tuberculata against paracetamol in experimentally induced liver toxicity in mice

  • Sana Department of Zoology, Islamia College University Peshawar, Pakistan
  • Sahfiq ur Rahman Department of Environmental Sciences, Shaheed Benazir Bhutto University, Sheringal Dir Upper, Pakistan
  • Muhammad Zahid Department of Zoology, Islamia College University Peshawar, Pakistan
  • Ayaz Ali Khan Department of Biotechnology, University of Malakand, Chakdara, 18800, Pakistan
  • Tariq Aziz School of Food and Biological Engineering, Jiangsu University, Zhenjiang China https://orcid.org/0000-0003-0905-8076
  • Zafar Iqbal Institute of Nursing Sciences, Khyber Medical University, Peshawar, Pakistan
  • Waqar Ali Department of Biotechnology, University of Malakand, Chakdara, 18800, Pakistan
  • Fehmida Farid Khan Department of Molecular Biology, Shaheed Zulfiqar Ali Bhutto Medical University Islamabad, Pakistan
  • Sumbal Jamil Rehman Medical Institute Peshawar, Khyber Pakhtunkhwa, Pakistan
  • Muhammad Shahzad School of Biological Sciences, Health and Life Sciences Building, University of Reading, Reading RG6 6AX, UK
  • Metab Alharbi Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
  • Abdulrahman Alshammari Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia

Abstract

Walnut Oil and Caralluma are edible and form part of the traditional medicine system in many countries. These are frequently used in traditional medicine as remedies to relieve a wide range of illnesses and health problems. Walnut Oil and Caralluma species have demonstrated anti-inflammatory, anti-nociceptive, antidiabetics, hepatoprotective, gastric mucosa protecting, antimalarial, antioxidant, anti-trypanosomal, appetite suppressant and cytotoxic activities. The current study was planned to study the impacts of 21 days’ oral administration of walnut oil and methanolic extract of Caralluma tuberculata on the levels of some liver-associated parameters and hematological parameters in paracetamol intoxicated mice. It was observed that paracetamol intoxication resulted in a considerable rise in serum ALT, cholesterol, triglycerides, Creatinine, and urea levels while a decrease in HDL level in comparison to mice normal control group (P<0.05). Serum ALT, cholesterol, triglycerides, creatinine, and urea levels of mice that were administered with walnut oil and methanolic extract of
C. tuberculata at the doses of (1 ml/kg, 2 ml/kg and 3 ml/kg body weight) were significantly lower when compared to toxic control mice group (P<0.05), While HDL level was significantly increased. The significant reduction had also been observed in the levels of serum parameters of mice group, which received standard hepato-protective drug i.e., vitamin C, at the dose of 8 mg/kg body weight (P<0.05). Based on these results, it was evident that liver toxicity caused by the paracetamol administration has recovered toward the normal range by the walnut oil and C. tuberculata extract. Therefore, the present study revealed that (walnut oil and
C. tuberculata) exhibit hepatoprotective activities in paracetamol intoxicated mice.

Published
2022-10-24
Section
Articles