Circ-POLA2-mediated miR-138-5p/SEMA4C axis affects colon cancer cell activities

  • YanDong Huang Department of Oncology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou City, Inner Mongolia Autonomous Region, 014017, China
  • QingYang Bai Department of Oncology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou City, Inner Mongolia Autonomous Region, 014017, China
  • HongBo Yu Practical Teaching Skills Center, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou City, Inner Mongolia Autonomous Region, 014017, China
  • YanRu Li Department of Oncology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou City, Inner Mongolia Autonomous Region, 014017, China
  • Hao Lu Department of Oncology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou City, Inner Mongolia Autonomous Region, 014017, China
  • HuiMin Kang Department of Oncology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou City, Inner Mongolia Autonomous Region, 014017, China
  • XueWei Shi Department of Oncology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou City, Inner Mongolia Autonomous Region, 014017, China
  • Kai Feng Department of Oncology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou City, Inner Mongolia Autonomous Region, 014017, China https://orcid.org/0000-0002-3162-8613
DOI: https://doi.org/10.18388/abp.2020_6457

Abstract

This study aimed to investigate the mechanism of circ-POLA2 in colon cancer (CC). Circ-POLA2, miR-138-5p, and SEMA4C levels in CC tissues and cells were recorded. The influences mediated by circ-POLA2, miR-138-5p or SEMA4C on cell proliferation, migration, invasion, and apoptosis were determined. The feedback loop of circ-POLA2/miR-138-5p/SEMA4C was surveyed. As measured, circ-POLA2 and SEMA4C were highly expressed, while miR-138-5p was poorly expressed. Meanwhile, circ-POLA2 could mediate SEMA4C through miR-138-5p targeting. Circ-POLA2 knockdown caused the blockade for cell activities, but this effect was alleviated by miR-138-5p inhibition or SEMA4C overexpression. Overall, circ-POLA2 is tumorigenic for CC through miR-138-5p/SEMA4C axis, which may provide a promising molecular target for CC therapy.

Published
2023-08-17
Issue
Vol. 70 No. 3 (2023)
Section
Articles

Copyright (c) 2023 YanDong Huang, QingYang Bai, HongBo Yu, YanRu Li, Hao Lu, HuiMin Kang, XueWei Shi, Kai Feng

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