Alpha-Mangostin ameliorates acute kidney injury via modifying levels of circulating TNF-α and IL-6 in glycerol-induced rhabdomyolysis animal model

Heba M. Eltahir; Hossein M. Elbadawy; Ali Alalawi; Ahmed J. Aldhafiri; Sabrin R.M.  Ibrahim; Gamal A. Mohamed; Abdel-Gawad S. Shalkami; Mohannad A.  Almikhlafi; Muayad Albadrani; Yaser Alahmadi; Mekky M. Abouzied; Maiiada H. Nazmy

doi:10.18388/abp.2020_6509

Heba M. Eltahir Department of Pharmacology and Toxicology, Division of Biochemistry, Taibah University, Medina, Kingdom of Saudi Arabia https://orcid.org/0000-0003-3308-1137
Hossein M. Elbadawy Department of Pharmacology and Toxicology, Taibah University, Medina, Kingdom of Saudi Arabia
Ali Alalawi Department of Pharmacology and Toxicology, Taibah University, Medina, Kingdom of Saudi Arabia
Ahmed J. Aldhafiri Department of Pharmacology and Toxicology, Taibah University, Medina, Kingdom of Saudi Arabia
Sabrin R.M. Ibrahim 3Preparatory Year Program, Batterjee Medical College, Jeddah 21442, Saudi Arabia; 4Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
Gamal A. Mohamed 5Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; 6Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut, Egypt
Abdel-Gawad S. Shalkami Department of Pharmacology and Toxicology, Faculty of Pharmacy, Al-Azhar University, Assiut, Egypt; 8Clinical Pharmacy Program, Faculty of Health Science and Nursing, Al-Rayan Colleges, Medina, Kingdom of Saudi Arabia
Mohannad A. Almikhlafi Department of Pharmacology and Toxicology, Taibah University, Medina, Kingdom of Saudi Arabia
Muayad Albadrani Department of Family and Community Medicine, College of Medicine, Taibah University, Medina, Kingdom of Saudi Arabia
Yaser Alahmadi Department of Clinical and Hospital Pharmacy, Taibah University, Medina, Kingdom of Saudi Arabia
Mekky M. Abouzied 1Department of Pharmacology and Toxicology, Division of Biochemistry, Taibah University, Medina, Kingdom of Saudi Arabia; 11Department of Biochemistry, Faculty of Pharmacy, Minia University, Minia, Egypt
Maiiada H. Nazmy Department of Biochemistry, Faculty of Pharmacy, Minia University, Minia, Egypt

DOI: https://doi.org/10.18388/abp.2020_6509

Abstract

Alpha mangostin (AM), isolated from G. mangostana, showed beneficial effects in several disorders due to its antioxidant and anti-inflammatory properties. Acute kidney injury (AKI) due to different etiologies can develop into severe complications, resulting in high mortality rates. In this work, AM is tested for its ability to alleviate AKI in glycerol-induced AKI rat model, where 30 Male Sprague-Dawley rats were assigned to a healthy group, glycerol-treated group and AM-treated group. Glycerol- and AM groups received a single dose of glycerol (per IM, 50% glycerol in saline, 8 ml/kg), whereas control group was injected with saline. AM treatment (a single daily dose, per IP, 175mg/kg) was accomplished for three days. Animals were executed to collect blood samples and kidney tissue for biochemical and histological examination. It was found that glycerol induced increase in serum creatinine, blood urea nitrogen (BUN), lipid peroxidation, serum magnesium, TNF-α and IL-6. It also induced renal edema and hypocalcemia along with histopathological renal damage. AM treatment improved renal histological features and alleviated increase in serum creatinine, BUN, serum magnesium, TNF-α and IL-6 levels, as well as renal edema and lipid peroxidation but did not affect serum calcium levels. This suggests AM as a potential therapeutic agent for treating AKI mainly via its antioxidant and anti-inflammatory properties.