PELP1 and SRC kinase as important molecules in the estrogen-mediated pathway in human testis and epididymis

Izabela Skibińska; Mirosław Andrusiewicz; Magdalena Jendraszak; Aleksandra  Żbikowska; Czesław Żaba; Małgorzata Kotwicka

doi:10.18388/abp.2020_6803

Izabela Skibińska Chair and Department of Cell Biology, Poznan University of Medical Sciences, Poznań, Poland https://orcid.org/0000-0002-7684-028X
Mirosław Andrusiewicz Chair and Department of Cell Biology, Poznan University of Medical Sciences, Poznań, Poland https://orcid.org/0000-0002-8781-3447
Magdalena Jendraszak Chair and Department of Cell Biology, Poznan University of Medical Sciences, Poznań, Poland
Aleksandra Żbikowska Chair and Department of Cell Biology, Poznan University of Medical Sciences, Poznań, Poland https://orcid.org/0000-0002-6891-8569
Czesław Żaba Chair and Department of Forensic Sciences, Poznan University of Medical Sciences, Poznań, Poland
Małgorzata Kotwicka Chair and Department of Cell Biology, Poznan University of Medical Sciences, Poznań, Poland

DOI: https://doi.org/10.18388/abp.2020_6803

Abstract

Background: Estrogens have pleiotropic mechanisms of action, and their cellular transduction pathways can modulate various proteins with differential tissue expression. Proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) is one such protein whose role seems important, although little is known about this protein. However, very little is known about the expression of modulators involved in the estrogen-mediated pathways in the tissues of the male reproductive tract. Methods: In this study, we obtained autopsy specimens of testis and epididymis from 13 men of Caucasian descent. Expression levels were analyzed for both estrogen receptors (ESR1 and ESR2) and their co-regulators, including PELP1 and kinase c-Src (SRC). Results: Protein expression was confirmed with western blot and immunocytochemistry techniques. The expression of both SRC and PELP1 was significantly higher in the testis compared to the epididymis (p=0.040 and p=0.002, respectively). Furthermore, a significant, positive correlation was observed between SRC and PELP1, regardless of tissue type p<0.0001, R=0.78). In the testis, PELP1 expression positively correlated with ESR1 expression

(p=0.367, R=0.6).

Conclusions: Our study suggests a possible relationship between PELP1, SRC, and ESR1 in the human testis and epididymis. This study makes a valuable contribution to the field of estrogen-mediated pathways in the male reproductive tract and describes trends of analyzed genes’ expression and presence. We think our results may open some new research directions of the estrogen signaling in the male reproductive system.