Isolation, preparation and investigation of leaf extracts of Aloe barbadensis for its remedial effects on tumor necrosis factor alpha (TNF-α) and interleukin (IL-6) by in vivo and in silico approaches in experimental rats

Iram Khurshaid; Sobia Ilyas; Nureen Zahra; Sohail Ahmad; Tariq Aziz; Fahad Al-Asmari; Sanaa Almowallad; Rehab F. Al-Massabi; Yasmene F. Alanazi; Aminah A. Barqawi; Roaa Mohammed Tahir Kassim; Abdulhakeem S Alamri; Majid Alhomrani; Manal Y Sameeh

doi:10.18388/abp.2020_6827

Iram Khurshaid Institute of Microbiology and Biotechnology The University of Lahore, Pakistan
Sobia Ilyas Institute of Microbiology and Biotechnology The University of Lahore, Pakistan
Nureen Zahra Institute of Microbiology and Biotechnology The University of Lahore, Pakistan
Sohail Ahmad Department of Poultry Production, University of Veterinary and Animal Sciences, Lahore, Pakistan
Tariq Aziz Department of Agriculture University of Iaonnina Arta, Greec
Fahad Al-Asmari Department of Food Science and Nutrition, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 400, Al-Ahsa, Saudi Arabia
Sanaa Almowallad Department of Biochemistry, Faculty of Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia
Rehab F. Al-Massabi Department of Biochemistry, Faculty of Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia
Yasmene F. Alanazi Department of Biochemistry, Faculty of Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia
Aminah A. Barqawi Department of Chemistry, Al-Leith University College, Umm Al-Qura University, Makkah 21955, Saudi Arabia
Roaa Mohammed Tahir Kassim Umm Al-Qura University, College of Science, Department of Biology, Makkah, Saudi Arabia
Abdulhakeem S Alamri Department of Clinical Laboratory Sciences, The Faculty of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Majid Alhomrani Department of Clinical Laboratory Sciences, The Faculty of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Manal Y Sameeh Department of Chemistry, Al-Leith University College, Umm Al-Qura University, Makkah 21955, Saudi Arabia

DOI: https://doi.org/10.18388/abp.2020_6827

Abstract

Aloe barbadensis is a stemless plant with a length of 60–100 cm with juicy leaves which is used for its remedial and healing properties in different suburbs of various countries. The present study was conducted to investigate the effect of A. barbadensis leaf extract (aqueous and ethanolic) in yeast induced pyrexia and acetic acid induced writhing in rat model to evaluate the antipyretic biomarkers and its phytochemical screening with computational analysis. For analgesic activity model 60 Albino rats (160–200 kg) were divided into four groups. Of the 4 groups, control consisted of 6 rats (Group I) treated with normal saline, standard comprised of 6 rats treated with drug diclofenac (Group I). Experimental groups consisted of 48 rats, treated with A. barbadensis ethanolic and aqueous leaf extracts at doses of 50 mg/kg, 100 mg/kg, 200 mg/kg, and 400 mg/kg (Group III. IV). For antipyretic activity group division was same as in analgesic activity. All groups were treated the same as in the analgesic activity except for the second group which was treated with paracetamol. In both antipyretic and analgesic activity at the dose of 400 mg/kg, group III showed significant inhibition. TNF–α and IL-6 showed significant antipyretic activity at a dose of 400 mg/kg. For molecular docking aloe emodin and cholestanol were used as ligand molecules to target proteins Tnf-α and IL-6. Acute oral toxicity study was performed. There was no mortality even at the dose of 2000 mg/kg. Quantitative and qualitative phytochemical screening was performed for the detection of various phytochemicals. Hence, A. barbadensis leaf extracts can be used in the form of medicine for the treatment of pain and fever.