Relative expression levels of growth hormone gene and growth rate in Indian major carp species

  • Shahid Sherzada Department of Zoology, Government College University Lahore, Pakistan
  • Muhammad Nauman Sharif Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
  • Qurban Ali Department of Plant Breeding and Genetics, University of the Punjab, Lahore, Pakistan
  • Saeed Akram Khan Department of Zoology, Government College University Lahore, Pakistan
  • Tawaf Ali Shah Department of Biotechnology, University of Okara, Punjab, Pakistan
  • Mohamed A. M. El-Tabakh Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
  • Tariq Aziz Laboratory of Animal Health, Food Hygiene and Quality, Department of Agriculture, University of Ioannina, 47132 Arta, Greece
  • Ghulam Nabi Institute of Nature Conservation Polish Academy of Sciences Krakow Poland
  • Metab Alharbi Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
  • Thamer H. Albekairi Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
  • Abdullah F Alasmari Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
DOI: https://doi.org/10.18388/abp.2020_6864

Abstract

The phenomenon of growth is a leading factor for aquaculture success. The uneven growth of major Indian carps (Labeo rohita, Catla catla, and Cirrhinus mrigala) is a serious issue in fish culture from an economic point of view. The growth hormone (GH) gene is crucial for selection in commercially cultivated fish species for better growth and production. Indian major carp (L. rohita, C. catla, and C. mrigala) are commonly cultured in Pakistan. The GH expression was examined using qPCR to understand growth in fish species better. Muscle tissue samples (n=480) from 160 individuals of the same age were collected from three species (L. rohita, C. catla, and C. mrigala). Individuals were divided into two groups (high-weight and low-weight groups), cultured under normal conditions. The housekeeping gene β-actin validated GH expression in fast and slow-growing fishes from the same species. Results showed that GH expression varies across species and fish specimens that overweight their counterpart feature have higher GH expression. A selection for overweight fish in the aquaculture breeding systems is preferable as those fish could inherit their genomics to the future cohort, enhancing production, and commercial profit for farmers. Comprehensive research about different growth genes and the environmental aspects that influence fish growth is mandatory. No work has been reported regarding the growth gene analysis of fish from Pakistan. This report was Pakistan’s first and baseline study regarding growth analysis of main culturable fish species at the molecular level.

Published
2023-11-14
Issue
Vol. 70 No. 4 (2023)
Section
Articles

Copyright (c) 2023 Shahid Sherzada, Muhammad Nauman Sharif, Qurban Ali, Saeed Akram Khan, Tawaf Ali Shah, Mohamed A. M. El-Tabakh, Tariq Aziz, Ghulam Nabi, Metab Alharbi, Thamer H. Albekairi, Abdullah F Alasmari

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