The relationship between EMG high frequency and low frequency band amplitude changes correlates with tissue inorganic phosphate levels

  • Małgorzata Habich Institute of Biochemistry and Biophysics Polish Academy of Sciences, Warsaw, Poland https://orcid.org/0000-0003-2631-0077
  • Bartosz Pawlinski Department of Large Animals with Clinic, Warsaw University of Life Sciences, Warsaw, Poland
  • Kamil Lorenc Department of Large Animals with Clinic, Warsaw University of Life Sciences, Warsaw, Poland
  • Maria Sady Department of Large Animals with Clinic, Warsaw University of Life Sciences, Warsaw, Poland
  • Katarzyna Siewruk Department of Large Animals with Clinic, Warsaw University of Life Sciences, Warsaw, Poland
  • Piotr Zielenkiewicz Institute of Biochemistry and Biophysics Polish Academy of Sciences, Warsaw, Poland
  • Zdzislaw Gajewski Department of Large Animals with Clinic, Warsaw University of Life Sciences, Warsaw, Poland
  • Jaroslaw Poznanski Institute of Biochemistry and Biophysics Polish Academy of Sciences, Warsaw, Poland
  • Leszek Paczek Department of Immunology, Transplantology, and Internal Diseases, Medical University of Warsaw, Warsaw, Poland
  • Pawel Szczesny Institute of Biochemistry and Biophysics Polish Academy of Sciences, Warsaw, Poland
DOI: https://doi.org/10.18388/abp.2020_6893

Abstract

Assessing inorganic phosphate levels seems crucial in deciphering the biochemical state of organisms or tissues. The concentration of inorganic phosphate in blood is an order of magnitude smaller than in tissues and, on top of that, it is dynamically used to fill temporary gaps in tissues. This is the reason blood inorganic phosphate level is considered a poor proxy for tissue levels. Therefore, tissue biopsy seems to be the dominant method when assessing inorganic phosphate levels for instance in muscles. In this study, we attempted to derive a non-invasive biomarker for phosphate tissue levels. We analyzed surface electromyography signals taken during 31P spectroscopy of leg muscles in five adult pigs. We induced hypophosphatemia via 20 minutes-long hyperventilation. It turned out that the proportion of the amplitude of the low frequency band and the high frequency band is significantly (p=0.002) correlated with the relative phosphate levels. The electromyographic signal did not correlate significantly with pCO2 levels in the blood, suggesting that the changes in the signal are a result of inorganic phosphate levels, not hyperventilation. The results might lead to the development of a real-time phosphate fluctuations measurement procedure.

Published
2023-10-18
Issue
Vol. 70 No. 4 (2023)
Section
Articles

Copyright (c) 2023 Małgorzata Habich, Bartosz Pawlinski, Kamil Lorenc, Maria Sady, Katarzyna Siewruk, Piotr Zielenkiewicz, Zdzislaw Gajewski, Jaroslaw Poznanski, Leszek Paczek, Pawel Szczesny

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